The game is scheduled to begin at 20:00 local time (16:00 GMT | 21:00 PKT).
Islamabad United are playing their third game in the competition. They had lost one and won one, the first game against Peshawar Zalmi when they chased down 190 runs.
The Gladiators will eye a big win against United tomorrow, to compensate the PSL final defeat.
However, United would also be looking forward to a victory to get back on the winning track.
Just In the news is that the IU management has replaced Sharjeel Khan with Rafatullah Mohmand for the rest of the tournament.
A nail-biting thriller is expected tonight on Sharjah Cricket Ground.
SQUADS
Islamabad United
Misbah-ul-Haq*, Amad Butt, Asif Ali, S Badree, SW Billings, BM Duckett, ST Finn, BJ Haddin, Hussain Talat, Imran Khalid, Khalid Latif, Mohammad Irfan, Mohammad Sami, Rumman Raees, Saeed Ajmal, Shadab Khan, Sharjeel Khan, DR Smith, SR Watson, Zohaib Khan
Quetta Gladiators
Sarfraz Ahmed*, Ahmed Shehzad, Anwar Ali, Asad Shafiq, Bismillah Khan, Hasan Khan, NL McCullum, Mahmudullah, TS Mills, Mir Hamza, Mohammad Nawaz (3), Noor Wali, NLTC Perera, KP Pietersen, RR Rossouw, Saad Nasim, Umar Amin, Umar Gul, LJ Wright, Zulfiqar Babar